Quantitative Western Blot Imaging

The multiplex fluorescent detection and chemiluminescent imaging capabilities of the Quantitative Western Blot Imaging FluorChem Q system provide a complete solution for quantitative Western blot imaging and analysis. The open platform is compatible with a wide variety of dyes and kits, providing the flexibility needed for multiple applications.

Six-position filter wheel provides UV and fluorescent imaging versatility Three excitation channels with specific wavelengths for multiplex fluorescent Western blot imaging Powerful fixed lens for rapid imaging speeds AlphaView® Q software saves imaging protocols and provides quantitative analysis tools

Multiplex Western Analysis

EXAMINE MULTIPLE PROTEINS SIMULTANEOUSLY

The FluorChem Q system allows quantitative detection of multiple proteins in a single assay, eliminating the need to strip and re-probe blots. Intensity values are easily normalized to a loading control to correct for inaccuracies.

Cy3 Labeled CEA Experimental Protein 1 Cy5 Labeled transferrin Experimental Protein 2 Fluorescein Labeled IgG Loading Control

Composite image of a three-color fluorescent Western blot, carcino embryonic antigen 
(CEA), transferrin and IgG were detected with fluorescently labeled antibodies, and 
imaged in three spectrally unique fluorescent channels. Imaging time: 24 seconds.

DETECT CO-MIGRATING PROTEINS

Overlapping proteins such as phosphorylated and unphosphorylated isoforms or other proteins with similar migration can be probed in the same blot. Our unique Channel Viewer feature allows visualization of individual bands in overlapping areas.

ULTRA-SENSITIVE PROTEIN DETECTION

The FluorChem Q system delivers the wide dynamic range and sensitivity essential to accurately detect subtle changes in protein levels.
 

Serial dilutions of transferrin detected by Western blot with a 
secondary Cy5 labeled antibody and imaged on the FluorChem Q 
system. A lower limit of 0.6 pg transferrin was detected.

 

ALPHAVIEW SOFTWARE FOR FLUORCHEM SYSTEMS

AlphaView Software is intuitive and easy to navigate for quantitative analysis. A pplication driven acquisition, experiment-based analysis protocols, and comprehensive annotation tools allow rapid generation of publicationready results and images.

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