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Laminar flow cabinet, complete analysis of cleaning and sterilization strategies 1

Pollution high-risk areas: these corners are secretly 'cultivating bacteria'
-Edge of high-efficiency filter: Aging sealing strips and loose screws can cause unfiltered air to seep in, forming a "pollution channel".
-Tabletop grooves and edges: When the pipette is knocked over or the culture medium drips, the liquid seeps into the gaps to form a biofilm, which cannot be killed by ordinary wiping
Surface of UV lamp: Dust on the surface of the lamp tube will absorb ultraviolet rays, resulting in a decrease of sterilization efficiency by more than 50%.
Return air vent and pre filter: If not cleaned for a long time, the accumulated dust becomes a "breeding ground" for microorganisms.
Oxytech
2、 The entire process of cleaning and sterilization: from "surface work" to "deep purification"
（1） Daily cleaning: 5 minutes a day to drive away "visible pollution"
-Power off for cooling: Turn off the power, wait for the fan to stop running (to avoid dust being raised by the airflow), and wear sterilized gloves for operation.
Physical cleaning:
-75% ethanol can be used in daily life, and it is recommended to use Octax sporicidal agent for accurate positioning, efficient sterilization, wiping of countertops, side walls, and lighting lampshades (note: pure alcohol should be avoided as high concentration alcohol evaporates quickly and may leave undissolved pollutants).
-For stubborn points, use a sterile cotton swab dipped in chemical disinfectant (recommended to use Octax sporicidal agent) to gently scrape, avoiding scratching the surface with steel wire balls or other materials.
-Use sterilized tweezers to thoroughly clean the corners and gaps with gauze strips, without leaving any "hygiene blind spots".
Key component care:
-Wipe the UV lamp with an anhydrous ethanol cotton ball to remove surface dust and fingerprints (oil absorbs UV rays).
-The pre filter (primary filter screen) should be taken out and rinsed with clean water every week, dried, and then reinstalled (reused 3-5 times before replacement to avoid fiber shedding and pollution).

（2） Deep sterilization: Once a week, eliminate 'invisible killers'
UV lamp sterilization:
-After cleaning, turn on the UV lamp, close the glass door, and irradiate for more than 30 minutes (ensuring that the UV intensity is ≥ 40 μ W/cm ², which can be detected by an radiometer, and if it is below the standard, the lamp needs to be replaced).
-Attention! UV lamps have a low efficiency in killing fungal spores, and it is recommended to cooperate with chemical disinfection.
Chemical disinfection "combination punch":
-Peracetic acid fumigation: Pour a 5% peracetic acid solution into a culture dish, place it in the center of the workbench, turn on the fan for 10 minutes, turn off the fan, and fumigate for 2 hours (suitable for scenarios with severe mold contamination, ventilate for 30 minutes after fumigation).
-Vaporized hydrogen peroxide (VHP): Using a dedicated generator, hydrogen peroxide molecules are allowed to penetrate into the gaps through low-temperature vaporization technology, achieving a 6-log killing effect in 30 minutes (suitable for frequently used pharmaceutical grade ultra clean benches).
-Attention: Metal parts should avoid contact with chlorine containing disinfectants (such as sodium hypochlorite) to prevent corrosion; Plastic materials should be used with caution with aldehyde disinfectants (such as formaldehyde), which may cause aging.
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