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Cleaning methods for laboratory carbon dioxide incubator

The incubator must be cleaned first to achieve disinfection and sterilization purposes, and many fungicides only have bactericidal activity on cleaned items. When cleaning, reagents that are chemically compatible with future disinfectants or fungicides must be used for cleaning and disinfection.
① Clean the surface of the box: first brush off the dust on the surface, then clean with a damp sponge or soft cloth, and then dry with a soft cloth. If there is dirt, clean with a low concentration cleaner and then wipe dry.
② Clean the inside of the box: Select a suitable disinfectant. All objects and surfaces must be cleaned, then rinsed with sterile water, and then wiped dry or air dried.
③ Cleaning glass doors: The cleaning agent used to clean the glass doors inside the incubator is the same as that used to clean the interior of the incubator. Then rinse with distilled water to remove residual cleaning agent, and finally dry the door with a soft cloth.
Disinfection and sterilization of carbon dioxide incubator
Disinfection refers to the physical or chemical means of killing microorganisms, but does not necessarily kill their spores. Sterilization refers to the killing of all microorganisms, and when the cells in the incubator are contaminated, sterilization measures need to be taken. Generally, disinfect the cells in the incubator once every 1-3 months on average without contamination. The three methods of disinfection and sterilization are liquid disinfectant, ultraviolet radiation, and heating.
① Liquid disinfectant: Choose a liquid disinfectant that is non corrosive to the incubator, and the disinfection effect of the liquid disinfectant is related to many factors. For example, the temperature of the site, contact time, pH, penetration ability, and reaction of organic matter. Some small changes in these factors may result in significant differences in the effectiveness of detergents. Therefore, even in very favorable circumstances, when it is ultimately required to be sterile, liquid detergents are not a reliable cleaning method.
② UV disinfection: Generally, clean it with distilled water first, and then irradiate it with the built-in UV lamp of the incubator for a day or with a portable UV lamp. The UV sterilization method is relatively simple as it can weaken the effectiveness of UV sterilization when obstructed or at a distance.
③ Heating: Damp heat and dry heat are considered methods of sterilization. In general environments, heating a high-pressure sterilizer to 121 ℃ is the method to quickly generate steam. However, carbon dioxide incubators cannot perform high-temperature and high-pressure sterilization, and can only be followed by 90 ℃ moist heat sterilization. However, 90 ℃ cannot achieve the permeability and high pressure effect brought by water vapor above 100 ℃ due to its low temperature, so the sterilization effect is also greatly reduced. Dry heat of 140 ℃ -180 ℃ can also play a sterilization role.
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